BiOptix ACCOLADE™
BiOptix Technology offers a new, patented approach to label-free analysis, called Surface Plasmon Enhanced interferometry (SPE). SPE yields better results with higher stability by simply and elegantly combining Surface Plasmon Resonance (SPR) with Common Path Interferometery. This integration leads to a robust system with improved stability, dynamic range and sensitivity, as well as reduced noise. Superior to classical interferometry, the ACCOLADE™ uses a single light beam that consists of both reference and sample phases that are recombined and the interference pattern converted into data. The system processes these signals and provides real-time results for faster decision making.
Currently, BiOptix offers a quad array chip enabling four features to be analyzed simultaneously for real-time binding comparison. Multiple features allow the experiment to be controlled for non-specific binding and/or evaluating more than one target at a time in parallel. In addition, the features are arranged to be close together (0.5mm) and share the same environment (fluid flow, temperature, etc.), thus binding data from all of the features are highly correlated.
The BiOptix ACCOLADE™ software is robust yet simple and user-friendly at the same time. The interface allows the user to build semi-complex, customized protocols using modular building blocks. These provide flexibility with minimal software training. For more complex protocols, ACCOLADE™ software allows authorized users access through the Building Block Compiler.
The software also provides a user the opportunity to follow the progress of the experiment while observing the data in real-time. Intuitive graphics show current conditions, protocol configuration, and instrument status.
The ACCOLADE™ software provides the user everything needed; tools for universal data export, control feature subtraction and multiple binding models to yield final-answer analysis.
ACCOLADE™ Specifications >>> read more»
System Ratings
Power 115VAC ±10%, 60Hz, 3A
Operational Temperature 10 to 35°C
Rated Pollution Degree 2
Elevation <2000m
Maximum Relative Humidity 75% for temperatures up to 30°C
50% Relative Humidity Above 35°C Indoor use
Analytical Performance
Optical Sensitivity 0.5 RU (Refractive index units: 1 RU = 10-6 RIU)
Baseline Drift < 0.3 RU/min
Mass on Biosensing Feature 0.5 pg/mm²
Analysis Time, Typical, < 10 min
Transducer Consumable Cassette
Minimum Sample Volume 250μl
Flow Rate Range 60 to 90 μl/min
Flow Cell Volume 6 μl
Flow Cell Sensing Area 2.4mm x 2.4mm, 4 separate features
Analysis Temperature Range 15 to 50°C
Ideal Ambient Temperature 15 to 25°C
In-line Degassing Standard
Kinetic Analysis
Association Rate Constant (ka) 103 to 107 M-1 s-1
Dissociation Rate Constant (kd) 10-5 to 0.5 s-1
Sample Concentration 10-3 to 10-11 M
Molecular Weight Detection >100 Da
Data Acquisition
Sampling Rate 2 to 20 Hz, selectable
Operating System Windows XP/Vista/Windows 7
System Interface/Transfer USB 2.0
Physical Parameters
Dimensions (W x D x H) 35cm x 50cm x 40cm
Weight 15 kg
Additional System Data (Available upon request)
Reproducibility Surface Stability
Intra-SensorChip Neutravidin-Biotin vs. Chemical Challenge
Inter-SensorChip Neutravidin vs. Chemical Challenge
Chip Charger Ligand
Immobilization Neutravidin vs. Drying
LOD Molecular Weight Dependence
